Catalog Number :BGK27548

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CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE.

The BioGems Murine CD40 Ligand Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a?solid phase immunoassay specially designed to measure CD40 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD40 Ligand. It contains NS0-expressed recombinant CD40 Ligand. The detection antibody is a biotinylated goat polyclonal antibody specific for CD40 Ligand. The kit is analytically validated with ready to use reagents.

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CD40是TNF受體家族的一員，是在B細(xì)胞，樹突狀細(xì)胞，單核細(xì)胞，胸腺上皮細(xì)胞上表達(dá)的細(xì)胞表面蛋白，在T細(xì)胞上表達(dá)水平低。CD40信號在B細(xì)胞的增殖和分化中起重要作用，并且對于免疫球蛋白（Ig）類型轉(zhuǎn)換（class switching）起關(guān)鍵作用。膜錨定的CD40配體幾乎完全只表達(dá)在活化的CD4 + T淋巴細(xì)胞上。未能表達(dá)CD40L會導(dǎo)致“高IgM免疫缺陷”，這是一種以未能產(chǎn)生IgG，IgA和IgE為特征的疾病。

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BioGems鼠CD40配體預(yù)包被ELISA（酶聯(lián)免疫吸附試驗(yàn)）試劑盒采用固相免疫測定法，專門設(shè)計(jì)用于檢測CD40配體，并在96孔微孔板上預(yù)包被CD40配體特異性小鼠單克隆抗體。它含有NS0表達(dá)的重組CD40配體。檢測抗體是CD40配體特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過分析驗(yàn)證，是即用型試劑。

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Product Details

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Components?組份

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Reproducibility?可重復(fù)性