產品英文名稱:WNT-5a,Mouse
產品中文名稱:鼠重組WNT-5a蛋白

WNT-5a,Mouse產品介紹:
wnt信號轉導通路因其啟動蛋白為Wnt蛋白而得名，在胚胎發(fā)育、細胞增殖及分化等方面發(fā)揮重要作用，其異常活化與人類多種腫瘤的發(fā)生密切相關。研究顯示，Wnt信號至少存在經典和非經典兩條不同的信號通路，而Wnt-5a屬于非經典Wnt信號通路，既往研究Ror2具有Wnt受體結構域樣結構。有研究認為Wnt-5a經由Ror2介導的 信號通路發(fā)揮作用。
Mouse WNT-5a蛋白含有356個氨基酸殘基。

WNT-5a,Mouse 產品特性:
? WNT-5a,Mouse活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞TCF報告鑒定，純度高
? 生物活性在共轉Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定
? WNT-5a,Mouse產品經過SDS-PAGE 和HPLC雙層鑒定純度大于85%

WNT-5a,Mouse產品英文簡述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.

? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (Milkels AJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.