產(chǎn)品英文名稱：Miltenyi CD34 MicroBeads, human
產(chǎn)品中文名稱：美天旎人CD34微珠試劑盒

Miltenyi CD34 MicroBead Kit 微珠試劑盒研究背景：
CD34抗原是一種單鏈跨膜糖蛋白表達(dá)于人類造血祖細(xì)胞，內(nèi)皮祖細(xì)胞，血管內(nèi)皮細(xì)胞，胚胎成纖維細(xì)胞，和一些細(xì)胞在胎兒和成人神經(jīng)組織。在CD34微珠套件包直接結(jié)合微珠到CD34抗體對CD34表達(dá)細(xì)胞的磁性標(biāo)記從外周血，臍帶血，骨髓，血液分離收獲，或分化的ES和iPS細(xì)胞。造血祖細(xì)胞，呈現(xiàn)在外周血中約0.05-0.2％的頻率，在臍帶血0.1-0.5％，并且在骨髓0.5-3％，可迅速而有效地豐富了。

美天旎人CD34微珠試劑盒產(chǎn)品介紹：
Components
2 mL CD34 MicroBeads, human:
MicroBeads conjugated to monoclonal mouseanti-human CD34 antibodies (isotype: mouseIgG1).
2 mL FcR Blocking Reagent, human:
Human IgG.
or
10 mL CD34 MicroBeads, human:
MicroBeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype: mouseIgG1).
10 mL FcR Blocking Reagent, human:
Human IgG.
Capacity
2 mL: For 2x10*9 total cells, up to 20 separations.
or
10 mL: For 10*10 total cells, up to 100 separations.
Product format
CD34 MicroBeads are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage
Store protected from light at 2?8 °C. Do not freeze. The expiration date is indicated on the vial label.

Miltenyi CD34 MicroBead Kit 微珠試劑盒應(yīng)用：
●正選擇或細(xì)胞表達(dá)人CD34抗原。
●分離造血祖細(xì)胞。
●分離內(nèi)皮祖細(xì)胞（EPCs）的。
●從分化的ES和iPS細(xì)胞培養(yǎng)中分離CD34+祖細(xì)胞
●在體外分化的研究。
●研究惡性血液病。

美天旎人CD34微珠試劑盒產(chǎn)品英文描述：
The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
peripheral blood (PBMCs)
bone marrow1
leukapheresis product
cord blood3
differentiated ES and iPS cell lines.
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.
Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice.