英文名稱：Recombinant Human Glial-Derived Neurotrophic Factor
中文簡稱：【GDNF】重組人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子

Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重組人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 細(xì)胞因子產(chǎn)品描述：

GDNF promotes the survival and differentiation of dopaminergic neurons in culture，and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF，two additional alternative transcripts encoding distinct proteins，referred to as astrocyte-derived trophic factors，have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子重組蛋白是在大腸桿菌中重組表達的，是由兩條非糖基化多肽鏈組成的同源二聚體。該重組蛋白包含有2 x 135個氨基酸，總分子量為30，360Da。該人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---大腸桿菌中重組表達

Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重組人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 細(xì)胞因子產(chǎn)品使用須知：
溶解： 提供的人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)的10mM檸檬酸鈉和150mM NaCl溶液中經(jīng)透析并凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 (GDNF)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重組人神經(jīng)膠質(zhì)源神經(jīng)營養(yǎng)因子 細(xì)胞因子產(chǎn)品英文概述：

Description 

Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.

Source   （來源）

Escherichia Coli.

Physical Appearance   （外觀）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl.

Solubility   （溶解性）

It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability  （穩(wěn)定性）

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity   （純度）

Greater than 98.0% as determined by

(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

Biological Activity  （生物活性）

The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg.