產品英文名稱: RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips
產品中文名稱: RoboSep™ 帶有過濾嘴的沒有CD16耗盡的人單核細胞富集試劑盒

RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips產品英文描述
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), which contains anti-CD16.

RoboSep™ 帶有過濾嘴的沒有CD16耗盡的人單核細胞富集試劑盒 Recommended for
The isolation of human monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection

RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips 產品組成
• EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL

RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips 保存方法
EASYSEP™ HUMAN MONOCYTE ENRICHMENT COCKTAIL WITHOUT CD16
DEPLETION
EASYSEP™ D MAGNETIC PARTICLES FOR MONOCYTES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

RoboSep™ 帶有過濾嘴的沒有CD16耗盡的人單核細胞富集試劑盒實驗資料
FACS Histogram Results Using EasySep® Human Monocyte Enrichment Kit Without CD16 Depletion

Starting with freshly prepared peripheral blood mononuclear cells, the CD14+ cell content of the enriched fraction typically ranges from 73% - 81%. Slightly lower CD14+ cell purities may be obtained from samples that contain a large number of CD16+ cells.

Typical EasySep™ Procedure for Human Cells

RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips 相關產品訂購信息
Stemcell  10406  CD14 Antibody, Clone MoP9, FITC-Conjugated  Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated 
Stemcell  07801  Lymphoprep™  Density Gradient Medium for the Isolation of Mononuclear Cells 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet Stemcell  18002  Stemcell Easy 50 EasySep™ Magnet  Immunomagnetic Column-Free Magnet Stemcell  19059  EasySep™ Human Monocyte Enrichment Kit  Immunomagnetic Negative Selection Kit 
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator
Stemcell  60004  Anti-Human CD14 Antibody, Clone M5E2  Mouse Monoclonal Antibody Against Human, Rhesus, Cynomolgus CD14 
Stemcell  60012  Anti-Human CD32 Antibody, Clone IV.3  Mouse Monoclonal Antibody Against Human CD32 
Stemcell  60018  Anti-Human CD45 Antibody, Clone HI30  Mouse Monoclonal Antibody Against Human, Chimpanzee CD45 
Stemcell  60026  Anti-Dextran Antibody, Clone DX1  Mouse Monoclonal Antibody Against Dextran   Against Human, Rhesus, Cynomolgus CD14   
For research use only. Not intended for human or animal diagnostic or therapeutic uses.