Murine β-NGF Pre-Coated ELISA Kit
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β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.
The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.
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β-NGF是與BDNF,NT-3和NT-4蛋白結(jié)構(gòu)相關(guān)的神經(jīng)營(yíng)養(yǎng)因子。這些蛋白屬于可能與穩(wěn)定二聚體結(jié)構(gòu)相關(guān)的半胱氨酸結(jié)家族生長(zhǎng)因子。 β-NGF是通過其受體β-NGFR傳導(dǎo)信號(hào)的強(qiáng)效神經(jīng)營(yíng)養(yǎng)因子,并且在感覺和交感神經(jīng)系統(tǒng)的發(fā)育和保存中起關(guān)鍵作用。 β-NGF還充當(dāng)B淋巴細(xì)胞的生長(zhǎng)和分化因子,并增強(qiáng)B細(xì)胞存活。
BioGems鼠β-NGF預(yù)包被ELISA(酶聯(lián)免疫吸附試驗(yàn))試劑盒采用固相免疫測(cè)定試劑盒,專門設(shè)計(jì)用于檢測(cè)β-NGF,其中96孔微孔板預(yù)包被β-NGF特異小鼠單克隆抗體NGF。它含有NS0表達(dá)的重組β-NGF。檢測(cè)抗體是對(duì)β-NGF特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過分析驗(yàn)證,是即用型試劑。
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Product Details
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Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
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Sensitivity: |
<1pg/ml |
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Range: |
31.2pg/ml-2000pg/ml |
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Specificity: |
Natural and recombinant Murine β-NGF |
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Uniprot: |
P01139 |
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Interference: |
No detectable cross-reactivity with other relevant proteins |
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Storage Conditions: |
Store the unopened product at 2 – 8 ℃. |
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Components組份
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Description |
Quantity |
Volume |
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Anti-Murine β-NGF Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Murine β-NGF Standard |
2 |
10 ng vial |
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Murine β-NGF Biotinylated antibody (100x) |
1 |
130 μL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 μL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
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Reproducibility?可重復(fù)性
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|
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Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
136 |
727 |
1326 |
153 |
839 |
1645 |
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Standard Deviation |
5.3 |
41.44 |
83.54 |
6.58 |
53.7 |
121.73 |
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Consistency (%CV) |
3.9 |
5.7 |
6.3 |
4.3 |
6.4 |
7.4 |
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